Colorimetric Determination of Free Fatty Acids in Biological Fluids.

Automated colorimetric determination of free fatty acids in biologic fluids.

HE MOST FREQUENTLY USED procedure for the determination of free fatty acids (FFA) in body fluids or biologic media is that of Dole (4). This method is based on the extraction of fatty acids with organic solvents followed by titration with dilute alkali. Despite improvements in specificity (3, 5, 6), this method is subject to error resulting from dissolved atmospheric carbon dioxide. When applie...

A more sensitive and stable colorimetric determination of free fatty acids in blood.

A sensitive method is proposed for the colorimetric determination of free fatty acids (FFA). Diphenylcarbazide containing diphenylcarbazone is used as the color developing reagent instead of diethyldithiocarbamate which was employed in the previous method (1965). J. Lipid Res. 6: 16-20). The present method was successfully applied to the determination of FFA present in as little as 40 microlite...

Semiautomated Method for the Colorimetric Determination of Plasma Free Fatty Acids.

A semiautomated procedure is described for the estimation of plasma free fatty acids. The method requires the preliminary preparation of a phospholipid-free plasma lipid extract which is then analyzed automatically by a colorimetric procedure based on the solubility of copper soaps in chloroform, with subsequent complexing of the copper with diethyldithiocarbamate and measurement of the extinct...

Determination and study the fatty acid contents and their seasonal variations by temperature of a dominant bivalve(Callista umbonella) of Haleh Creek

In this study fatty acid contents, because of the importance in human’s life, and their seasonal variations of a dominant bivalve of Haleh Creek were determined for the first time. Fatty acid identification was done by GC-MS (Gas Chromatography–Mass Spectrometry) method. After collecting and dissecting the species from the shells, samples weighed and frozen for further experiments. All the samp...

Colorimetric Ultramicro Method for the Determination of Free Fatty Acids.